Tóm tắt

Objectives: To investigate the expression of beta 7 integrin on B and T lymphocytes using a flow cytometry system. Subjects and methods: We analyzed immunological parameters such as B and T lymphocyte population as well as B and T cell subpopulations expressing beta 7 integrin molecules by flow cytometry on the two groups of children with and without response to the Rotavirus vaccine. Results: The number of peripheral blood lymphocytes of the non-response group was statistically significantly lower than that of the response group ( ± SD: 4.89 ± 1.71 vs 5.77 ± 1.46 million/mL, p = 0.04). The number of T cells (CD3+) of the non-response group was statistically significantly lower than that of the response group ( ± SD: 1.65 ± 0.61 vs 2.05 ± 0.64 million/mL, p = 0.018). The frequency and number of T-cells expressing beta 7 integrin of the non-response group were statistically significantly lower than those of the response group (Frequency ( ± SD): 17.48 ± 2.89 vs 19.44 ± 3.74%, p = 0.029; Quantity ( ± SD): 0.87 ± 0.41 vs 1.13 ± 0.37 million/mL, p = 0.014). There was no statistically significant difference between 2 groups for B-cell-related parameters in peripheral blood such as the number and frequency of total B cells and the B-cell subpopulation expressing beta 7 integrin in peripheral blood. Conclusion: The group of children not responding to the Rotavirus vaccine was statistically significantly lower than that of the responding group for the following parameters: Total lymphocyte count; frequency and number of the subpopulation of T-lymphocytes expressing 7 beta integrin in peripheral blood. * Keywords: Rotavirus vaccine; Beta 7 integrin; β7- integrin; B lymphocytes; T lymphocytes.

Abstract

Objectives: To investigate the expression of beta 7 integrin on B and T lymphocytes using a flow cytometry system. Subjects and methods: We analyzed immunological parameters such as B and T lymphocyte population as well as B and T cell subpopulations expressing beta 7 integrin molecules by flow cytometry on the two groups of children with and without response to the Rotavirus vaccine. Results: The number of peripheral blood lymphocytes of the non-response group was statistically significantly lower than that of the response group ( ± SD: 4.89 ± 1.71 vs 5.77 ± 1.46 million/mL, p = 0.04). The number of T cells (CD3+) of the non-response group was statistically significantly lower than that of the response group ( ± SD: 1.65 ± 0.61 vs 2.05 ± 0.64 million/mL, p = 0.018). The frequency and number of T-cells expressing beta 7 integrin of the non-response group were statistically significantly lower than those of the response group (Frequency ( ± SD): 17.48 ± 2.89 vs 19.44 ± 3.74%, p = 0.029; Quantity ( ± SD): 0.87 ± 0.41 vs 1.13 ± 0.37 million/mL, p = 0.014). There was no statistically significant difference between 2 groups for B-cell-related parameters in peripheral blood such as the number and frequency of total B cells and the B-cell subpopulation expressing beta 7 integrin in peripheral blood. Conclusion: The group of children not responding to the Rotavirus vaccine was statistically significantly lower than that of the responding group for the following parameters: Total lymphocyte count; frequency and number of the subpopulation of T-lymphocytes expressing 7 beta integrin in peripheral blood. * Keywords: Rotavirus vaccine; Beta 7 integrin; β7- integrin; B lymphocytes; T lymphocytes.